HPLC ≥99.0%|CAS-TRACEABLE|SPPS-READY|CoA PER BATCH

GL Biochem

Shanghai amino acid and research compound supplier · Pharma-grade reagents
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// Amino acid building blocks · Custom synthesis · Pharma-grade

Fmoc & Boc amino acids, with full CAS & analytical traceability.

Direct manufacturer of Fmoc-protected and Boc-protected amino acid building blocks for solid-phase peptide synthesis (SPPS), plus custom peptide synthesis services and analytical-grade research compounds.

Each catalog entry carries a CAS reference, molecular formula, MW and HPLC purity ≥99.0%. Fmoc and Boc amino acids · Custom synthesis · Pudong facility.

HPLC ≥99.0% SPPS-ready CAS-traceable CoA per batch −20°C dry

First-order discount

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SAVE15
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Catalog size
12 amino acids & reagents
Purity
≥99.0% HPLC verified
Custom synthesis
Available · enquire
Shipping
China · Worldwide express
SECTION 02 // CATALOG

Amino acid building blocks

12 compounds · CAS / MW / formula per entry
Cat. No.CompoundCASMWPurityMOQPriceOrder
GL-AA-0001Fmoc-Ala-OH
C18H17NO4
35661-39-3311.3399.5%100g$19Order →
GL-AA-0002Fmoc-Arg(Pbf)-OH
C28H38N4O7S
154445-77-9648.7899.0%25g$40Order →
GL-AA-0003Fmoc-Asn(Trt)-OH
C38H32N2O5
132388-59-1596.6899.0%25g$50Order →
GL-AA-0004Fmoc-Asp(OtBu)-OH
C23H25NO6
71989-14-5411.4599.5%100g$27Order →
GL-AA-0005Fmoc-Cys(Trt)-OH
C36H31NO4S
103213-32-7585.799.0%25g$37Order →
GL-AA-0006Fmoc-Gln(Trt)-OH
C39H34N2O5
132327-80-1610.799.0%25g$48Order →
GL-AA-0007Fmoc-Glu(OtBu)-OH
C24H27NO6
71989-18-9425.4899.5%100g$25Order →
GL-AA-0008Fmoc-Gly-OH
C17H15NO4
29022-11-5297.3199.5%100g$22Order →
GL-AA-0009Fmoc-His(Trt)-OH
C39H33N3O4
109425-51-6619.799.0%25g$46Order →
GL-AA-0010Fmoc-Ile-OH
C21H23NO4
71989-23-6353.4199.5%100g$16Order →
GL-AA-0011Fmoc-Leu-OH
C21H23NO4
35661-60-0353.4199.5%100g$21Order →
GL-AA-0012Fmoc-Lys(Boc)-OH
C26H32N2O6
71989-26-9468.5599.0%100g$26Order →

Bulk pricing on quantities > 100g. Custom synthesis available — full price list and SDS on the catalog site.

SECTION 03 // QUALITY CONTROL

Analytical specifications

Method
RP-HPLC, UV-DAD
Reverse-phase chromatography establishes purity. Quantitation by external standard.
Identity
¹H-NMR / MS
Where applicable, supplemented by mass spectrometry [M+H]⁺ confirmation.
Purity
≥99.0%
Per published reference. Optical rotation [α]ᴅ within published range.
Loss on drying
≤ 1.0%
Karl-Fischer titration confirms moisture content within specification.
Heavy metals
≤ 10 ppm
Per ICH Q3D guidance for elemental impurities in reagent-grade materials.
Storage
−20°C, dry
Long-term stability validated. Inert headspace recommended after opening.
Solubility
DMF, DMSO, DCM
Standard SPPS solvents. Per-compound solubility on individual SDS.
Documentation
CoA + SDS
Per-batch certificate of analysis with each shipment. SDS on the catalog page.
Quality & compliance reference

The QC pipeline aligns with ICH guidance and standard pharmacopoeial methodology. Documentation is auditable and prepared for institutional and pharmaceutical purchasing committees.

ICH Q2 (R1)

Validation of analytical procedures — HPLC, NMR, MS methods documented.

ICH Q3D

Elemental impurity limits — ICP-MS data on file for each batch on request.

USP <1226>

Verification of compendial procedures applied to suitable monographs.

ISO 9001

Quality management system covers all manufacturing and QC operations.

Standards aligned ICH Q2(R1) ICH Q3D ICH Q3C ICH Q7 ICH Q1A(R2) USP <71> USP <85> USP <61> USP <62> USP <788> USP <1226> EP 2.6.14 EP 2.9.20 JP 4.01 ISO 9001:2015 ISO/IEC 17025
Analytical record · Lot B-2026-0428-001

Reference standard — full analytical profile

Every batch is released against a written specification with identity (LC-MS), assay (RP-HPLC), endotoxin (LAL), water (Karl-Fischer), residual solvents (GC-headspace) and microbial limits — recorded on a per-lot Certificate of Analysis.

RP-HPLC trace · purity
Fmoc-Ala-OH
Rt 12.84 min
99.74%
Rt 12.84 min · 99.74% · API peak Rt 18.7 · 0.21% impurity RP-HPLC C18 · 0.1% TFA · MeCN/H₂O · 220 nm Phenomenex Luna 250×4.6 mm · 5 μm
ESI-Q-TOF MS · identity
[M+H]⁺ confirmed
m/z 100 – 6000
±0.3 Da
[M+H]⁺ · base peak [M+2H]²⁺ [M+Na]⁺ ESI-Q-TOF · positive ion · m/z 100 – 6000 Bruker Compact · ±0.5 Da
Purity (HPLC)
99.74%
UV-DAD 220 nm · A%
Identity (MS)
[M+H]⁺ ✓
±0.3 Da of theoretical
Endotoxin
< 0.05 EU/mg
LAL kinetic · USP <85>
Water (KF)
2.3% w/w
Coulometric titration
Certificate of Analysis
Fmoc-Ala-OH · Lot B-2026-0428-001
Mfg 2026-04-28
Exp 2029-04-28
CompoundFmoc-Ala-OH
CAS number35661-39-3
Molecular formulaC18H17NO4
Molecular weight311.33 g/mol
AppearanceWhite to off-white lyophilised cake
SolubilityFreely soluble in water (≥ 5 mg/mL); soluble in 0.1 M acetic acid
Identity (ESI-Q-TOF)[M+H]⁺ ±0.3 Da of theoretical · matches reference spectrum
Assay (RP-HPLC, A%)99.74% (Spec ≥ 98.0%)
Net peptide content88.4% w/w (AAA)
Endotoxin (LAL kinetic)< 0.05 EU/mg · USP <85> (Spec < 5.0 EU/mg)
Microbial — TAMC< 10 CFU/g · USP <61> (Spec < 100)
Microbial — TYMC< 10 CFU/g · USP <61> (Spec < 10)
Specified microorganismsAbsent · USP <62> (S. aureus / E. coli / P. aeruginosa)
Water content (KF)2.3% w/w (Spec ≤ 5.0%)
Acetate counter-ion7.8% w/w · ion chromatography
TFA residual< 1.0% · ¹⁹F-NMR
Heavy metals (total)< 10 ppm · ICH Q3D · ICP-MS
FormLyophilised, sterile-filtered (0.22 μm), N₂-purged amber glass vial
Backbone topology · structural reference
Fmoc-Ala-OH · primary structure
Fmoc-SPPS · TFA cleavage · RP-HPLC purified
H₂N R₁ R₂ R₃ R₄ R₅ O O O O O OH Cα Cα Cα Cα Cα Cα Generic peptide backbone — amide bonds, side chains R₁–Rₙ — synthesised by Fmoc-SPPS, cleaved with TFA cocktail K, RP-HPLC purified
— Reference standard programme · synthesis → QC → release —

Endotoxin · microbial limits · elemental impurities

USP <85> · <61> · <62> · ICH Q3D
Bacterial endotoxins
< 0.05 EU/mg
LAL kinetic chromogenic · USP <85>
Total aerobic count
< 10 CFU/g
USP <61> · TAMC
Total yeast / mould
< 10 CFU/g
USP <61> · TYMC
Specified microorganisms
absent
USP <62> · S. aureus / E. coli / P. aeruginosa
Heavy metals (total)
< 10 ppm
ICH Q3D · ICP-MS · Pb/Cd/As/Hg
Sterility · USP <71>
no growth
14-day · FTM + SCDM · membrane filtration
Acetate content
7.8% w/w
Counter-ion · ion chromatography
TFA residual
< 1.0%
Trifluoroacetate · ¹⁹F-NMR

Residual solvents — ICH Q3C(R8)

GC headspace · FID
Class Solvent PDE / limit Result Status
Class 1Benzene2 ppmnot detected● pass
Class 1Carbon tetrachloride4 ppmnot detected● pass
Class 2Acetonitrile410 ppm38 ppm● pass
Class 2Dichloromethane600 ppm21 ppm● pass
Class 2N,N-DMF880 ppm112 ppm● pass
Class 3Ethanol5000 ppm86 ppm● pass
Class 3Ethyl acetate5000 ppm42 ppm● pass
Container · closure system
2 mL Type I amber borosilicate glass vial · 13 mm bromobutyl-rubber stopper (B2-40, FluroTec® coated) · aluminium flip-off seal · headspace purged with dry N₂. Tested per USP <1660> · <381> · <671> for moisture vapour transmission.
Particulate matter · USP <788>
Method 1 · light obscuration. ≥ 10 μm: 18 / mL (limit ≤ 6000); ≥ 25 μm: 1 / mL (limit ≤ 600). Reconstituted 1 mg/mL in water-for-injection. EP 2.9.19 cross-validated. Pass.
Bioactivity · receptor binding
In-vitro binding assay vs. cognate reference standard. EC₅₀ relative potency 96 – 104% (n=3). cAMP accumulation HEK-293 stable line · LANCE® TR-FRET · 4-parameter logistic fit. Within ±10% spec.

Real-time stability data — 36 months @ −20 °C

ICH Q1A(R2) · long-term + accelerated arms
t (mo) Storage Assay (HPLC) Δ vs t₀ Endotoxin Water (KF) Status
0−20 °C, dry99.74%reference< 0.05 EU/mg2.3%● t₀
3−20 °C, dry99.73%−0.01%< 0.05 EU/mg2.3%● within spec
6−20 °C, dry99.71%−0.03%< 0.05 EU/mg2.4%● within spec
12−20 °C, dry99.65%−0.09%< 0.05 EU/mg2.5%● within spec
24−20 °C, dry99.52%−0.22%< 0.05 EU/mg2.6%● within spec
36−20 °C, dry99.41%−0.33%< 0.07 EU/mg2.7%● within spec
Test points t = 0, 3, 6, 9, 12, 18, 24, 30, 36 mo. Acceptance criterion: assay ≥ 95.0% of t₀, endotoxin < 5.0 EU/mg, water ≤ 5.0% w/w. Accelerated arm 25 °C / 60% RH passed without specification deviation through 6 months.

Analytical method strings

validated per ICH Q2(R1) · linearity / accuracy / precision / specificity / LOD-LOQ
RP-HPLC · purity / assay
Phenomenex Luna C18(2), 250 × 4.6 mm, 5 μm. Mobile A: 0.1% TFA in H₂O · B: 0.1% TFA in MeCN. Gradient 20 → 80% B over 30 min, hold 5 min. Flow 1.0 mL/min. Column 35 °C. UV-DAD 220 nm. Injection 20 μL · 1 mg/mL.
ESI-Q-TOF MS · identity
Bruker Compact Q-TOF · positive ion. m/z 100 – 6000. Capillary 4500 V. Nebuliser 1.6 bar. Dry gas 8.0 L/min @ 200 °C. Mass accuracy ±0.5 Da of theoretical [M+H]⁺ / [M+2H]²⁺ / [M+3H]³⁺.
LAL kinetic chromogenic · endotoxin
Charles River Endosafe nexgen-PTS · USP <85> / EP 2.6.14 / JP 4.01. Sensitivity 0.005 – 50 EU/mL. MVD calculated from CSE. Acceptance: spike recovery 50 – 200%; r ≥ 0.980 over standard curve.
Karl-Fischer · water content
Metrohm 870 KF Titrino plus · coulometric. Anode HYDRANAL Coulomat AG. Sample 50 – 100 mg, headspace-free. Acceptance ≤ 5.0% w/w. Reported to 0.1% precision. Cross-checked vs. TGA.
ICP-MS · elemental impurities
Agilent 7900 ICP-MS · ICH Q3D Option 1. Sample digestion: closed-vessel microwave (HNO₃ + H₂O₂). Target panel: Pb, Cd, As, Hg + Class 2A/2B. RSD ≤ 5%. Recovery 70 – 150% of spiked control.
AAA · amino-acid analysis
Hitachi L-8900 · post-column ninhydrin. Hydrolysis 6 N HCl · 110 °C · 24 h, vacuum-sealed, phenol added. Reports residue ratios + net peptide content (anchor for assay value). Cross-validated vs. nitrogen elemental analysis.
Quality system
ISO 9001:2015 certified
Certificate CN-11/Q-2417 · TÜV Rheinland
Test laboratory
ISO/IEC 17025 accredited
Scope: chemical & biological testing
Manufacturing
ICH Q7-aligned API
Process validation 3 consecutive batches
Compendial cross-ref
USP-NF · EP · JP · ChP
Specifications mapped to monograph chapters
Place an order against this catalog
Bulk pricing, milligram–gram scale, full CoA on every shipment
Order online →
SECTION 04 // ORDER

Order online

All orders are placed online. Browse catalog, select compounds and quantities, apply your discount code at checkout. Custom synthesis enquiries via the technical contact below.

Order online →
First-order discount · code SAVE15 · 15% off
// Technical support
WhatsApp
+1 (515) 983-8220
Email
info@glbiochem.net